The electrophoretic behaviour of mitochondria from kidney and liver of rats

Publisher Website
Google Scholar
Abstract
The electrophoretic mobility values for mitochondria prepared from rat kidney and liver measured in 0.125 [image]-potassium chloride-0-02 [image]-tris, pH 7.4, are: -0.78 (S.E.M.[plus or minus]0-02)[mu]/sec./v/cm. (29 experiments) and - 1.06(S.E.M[plus or minus]0-01)[mu]/sec./v/cm. (21 experiments) respectively. These mobility values are unaffected by washing and spontaneous swelling at 25[degree], indicating a stable electrokinetic surface. The mobility of rat-kidney mitochondria is unaffected by thyroxine-induced swelling, or by the state of hydration of the rat. pH-mobility curves show similar surface ionogenic groups for kidney and liver mitochondria; their isoelectric points are pH 3.9 and pH 4.4 respectively.