Biosynthesis of cyclosporin A: partial purification and properties of a multifunctional enzyme from Tolypocladium inflatum
- 11 February 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (3) , 550-553
- https://doi.org/10.1021/bi00351a005
Abstract
An enzyme fraction most probably involved in the biosynthesis of cyclosporin A was purified 540-fold from Tolypocladium inflatum. The enzyme was capable of forming covalent enzyme-substrate complexes and catalyzed the ATP-pyrophosphate exchange reactions dependent on the unmethylated constituent amino acids of cyclosporin A. Evidence was obtained that covalent binding of substrate amino glycine residues with S-adenosyl-L-methionine was demonstrated. De novo synthesis of cyclosporin A was under the consumption of ATP and S-adenosyl-L-methionine. This cyclodipeptide represents a partial sequence of cyclosporin A. Molecular mass determinations revealed the enzyme activity to be lying in the range of about 700 kDa.This publication has 1 reference indexed in Scilit:
- Cell-free synthesis of the depsipeptide beauvericin.The Journal of Antibiotics, 1983