Structure of genes for human growth hormone and chorionic somatomammotropin.
- 1 September 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (9) , 4294-4298
- https://doi.org/10.1073/pnas.76.9.4294
Abstract
A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences were identified by hybridization to cloned c[complementary]DNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2-3 kb was ligated to [bacteriophage] .lambda.gt WES .cntdot. .lambda.B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains 3 intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.This publication has 28 references indexed in Scilit:
- Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun CollectionsScience, 1978
- Nucleotide sequence homology at 12 intron–exon junctions in the chick ovalbumin geneNature, 1978
- Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.Proceedings of the National Academy of Sciences, 1978
- The rabbit β-globin gene contains a large insert in the coding sequenceCell, 1977
- Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.Proceedings of the National Academy of Sciences, 1977
- A physical map of the DNA regions flanking the rabbit β-globin geneCell, 1977
- Packaging recombinant DNA molecules into bacteriophage particles in vitro.Proceedings of the National Academy of Sciences, 1977
- In vitro packaging of a λ Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1Gene, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Efficient transcription of RNA into DNA by avian sarcoma virus polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976