Mitochondrial and Chloroplast Phage-Type RNA Polymerases in Arabidopsis
- 8 August 1997
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 277 (5327) , 809-811
- https://doi.org/10.1126/science.277.5327.809
Abstract
In addition to the RNA polymerases (RNAPs) transcribing the nuclear genes, eukaryotic cells also require RNAPs to transcribe the genes of the mitochondrial genome and, in plants, of the chloroplast genome. The plant Arabidopsis thaliana was found to contain two nuclear genes similar to genes encoding the mitochondrial RNAP from yeast and RNAPs of bacteriophages T7, T3, and SP6. The putative transit peptides of the two polymerases were capable of targeting fusion proteins to mitochondria and chloroplasts, respectively, in vitro. The results indicate that the mitochondrial RNAP in plants is a bacteriophage-type enzyme. A gene duplication event may have generated the second RNAP, which along with the plastid-encoded eubacteria-like RNAP could transcribe the chloroplast genome.Keywords
This publication has 24 references indexed in Scilit:
- Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium albumNucleic Acids Research, 1997
- An ancestral mitochondrial DNA resembling a eubacterial genome in miniatureNature, 1997
- Quartet Puzzling: A Quartet Maximum-Likelihood Method for Reconstructing Tree TopologiesMolecular Biology and Evolution, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Truncated presequences of mitochondrial F1-ATPase β subunit from Nicotiana plumbaginifolia transport CAT and GUS proteins into mitochondria of transgenic tobaccoPlant Molecular Biology, 1994
- Crystal structure of bacteriophage T7 RNA polymerase at 3.3 Å resolutionNature, 1993
- The rapid generation of mutation data matrices from protein sequencesBioinformatics, 1992
- Isolation and characterization of mutant bacteriophage T7 RNA polymerasesJournal of Molecular Biology, 1992
- Replication and Transcription of Plastid DNAPublished by Elsevier ,1991
- Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7Cell, 1987