INDUCTION OF CYTOTOXICITY OF LYMPHOCYTES FROM TOLERANT DONORS BY ANTIBODIES TO TARGET CELL ALLOANTIGENS

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Abstract
SUMMARY Alloantibodies from Campbell ducks which induce rejection of tolerated skin allografts from Peking ducks in vivo were studied for their capacity to induce cell-mediated cytotoxic reactions in vitro. In the presence of heat-inactivated alloantiserum, Campbell lymphocytes from tolerant ducks became eytotoxic to Peking target cells. Cytotoxicity was assayed either as release of 51Cr from labeled Peking erythrocytes or as reduction the number of fibroblasts growing in microtest plates. The sera were not eytotoxic without addition of lymphocytes, and normal Campbell sera with lymphocytes had no effect. Syngeneic or xenogeneic (chicken) target cells were not affected. Antiseruminduced cytotoxicity was also obtained with lymphocytes from Campbell ducks not tolerant to Peking alloantigens or with human lymphocytes. In 11 of 15 immune sera studied, a good correlation was found between their eytotoxic activity in vitro and their graft-rejecting capacity in vivo. No correlation was observed between the capacity of the sera to induce cell-mediated cytotoxicity in vitro and their capacity to produce Arthus-like reactions in vivo.

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