Some problems in the use of the Coulter counter for erythrocyte total counts and volume distribution
- 1 March 1968
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 21 (2) , 179-182
- https://doi.org/10.1136/jcp.21.2.179
Abstract
Normal erythrocytes were subject to swelling and some hemolysis when suspended in a NaCl-formaldehyde-Na2EDTAdiluentof pH 4.9 for routine blood-cell counting in the Coulter counter. A phosphate-buffered saline of pH 7.4 was substituted and has proven satisfactory for total erythrocyte counts. Polyvinyltoluene latex was unsuitable for volume calibration of the counter, because of aggregation of the particles in saline. Ragweed pollen grains gave a calibration factor that agreed well with that for fresh erythrocytes. Recent batches of Dad''s Erythro-Trol (a suspension of fixed erythrocytes) were satisfactory for both total counts and volume calibration.This publication has 8 references indexed in Scilit:
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