Ribosyl-cis-zeatin in a Leucyl Transfer RNA Species from Peas

Abstract
TRNA6Leu in Pisum sativum seed has been purified. This tRNA species contains a cytokinin-active nucleoside and accounts for approximately 7% of the total cytokinin activity in acid hydrolysates of pea tRNA. The cytokinin has been identified as ribosyl-cis-zeatin, 6-(4-hydroxy-3-methyl-cis-2-butenylamino) -9-β-d-ribofuranosylpurine.