PLATELET MONOAMINE-OXIDASE .1. EFFECT OF TEMPERATURE, ANTICOAGULANT, AND CENTRIFUGATION TECHNIQUE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 16  (11) , 1077-1083
Abstract
Platelet yield and monoamine oxidase (MAO) activity per platelet in platelets obtained by the method of Corash, which isolates essentially all platelets from whole blood, and by a single differential centrifugation of whole blood, were compared. Platelets recovered by 1 spin in a swinging bucket rotor appeared to be a homogeneous population containing a reliable representation of the platelet population obtained by the Corash method since the MAO specific activity yielded by the 2 methods was not significantly different. The effect of the anticoagulant, temperature and type of rotor on platelet recovery from blood and MAO activity/platelet were also studied. The MAO and plasma amine oxidase (PAO) activities obtained from blood anticoagulated with EDTA were significantly greater than those found when acid-citrate-dextrose was the anticoagulant. Blood centrifuged in a swinging bucket rotor at room temperature yielded 80-90% of total platelets as compared to the 45-65% recovered with a fixed-angle rotor at 0-4.degree. C. The most efficient method of platelet isolation was a 2 1/2-min room-temperature spin at 600 .times. g with a swinging bucket rotor using EDTA as anticoagulant.