On the use of excision repair defective cells in the wing somatic mutation and recombination test inDrosophila melanogaster
- 1 January 1990
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 16 (4) , 225-237
- https://doi.org/10.1002/em.2850160403
Abstract
Ten chemical mutagens were tested in the wing somatic mutation and recombination test in Drosophila melanogaster. This assay makes use of genetic markers expressed on the wing of adult flies. Larvae which are trans‐heterozygous for mwh (multiple wing hairs) and flr (flare) were fed with the compounds either acutely (2, 4, or 6 hr) or chronically (48 or 72 hr), or were treated by inhalation (1 hr). Genetic changes induced in the somatic cells of the wing imaginal discs lead to the formation of mutant clones on the wing (mwh and/or flr). Single spots are produced by point mutation, chromosome breakage, and mitotic recombination; twin spots are produced exclusively by mitotic recombination. All 10 mutagens belonging to different chemical classes were clearly positive in this assay. However, the choice of the optimal treatment conditions (concentration of compound, duration of treatment, age of larvae at treatment) is essential. Eight of the compounds were also tested in excision repair defective cells by introducing the mei‐9L1 mutation into the test system. This seems not to improve the detection capacity of the assay, mainly because only small spots are found in excision repair defective wings. In addition, the frequencies of spots in these wings are enhanced four to five times, which makes the scoring more tedious. For these and other practical reasons the use of this specific cross is not recommended in the wing spot test for routine screening purposes.Keywords
This publication has 39 references indexed in Scilit:
- Genotoxicity of aniline derivatives in various short-term testsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1989
- Genotoxicity testing of antiparasitic nitrofurans in the Drosophila wing somatic mutation and recombination testMutagenesis, 1989
- A cell-cycle stage-related chromosomal X-ray hypersensitivity in larval neuroblast of Drosophila mei-9 and mei-41 mutants suggesting defecting DNA double-strand break repairMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1989
- The relationship between reaction kinetics and mutagenic action of monofunctional alkylating agents in higher eukaryotic systemsIV. The effects of the excision-defective mei-9L1 and mus(2)201D1 mutants on alkylation-induced genetic damage in DrosophilaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1985
- Somatic mutation and recombination test in Drosophila melanogasterEnvironmental Mutagenesis, 1984
- Genotoxic activity of the anti-cancer drug methotrexate in somatic cells of Drosophila melanogasterMutation Research Letters, 1983
- Mutagen-sensitive mutants in Drosophila melanogasterMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Sensitivity of drosphila mutants to chemical carcinogensMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Genetic control of mutagen sensitivity in Drosophila melanogasterMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Detection of somatric recombination and mutation in Drosophila a method for testing genetic activity of chemical compoundsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974