A Rapid Method for the Purification of Immunoglobulin M (IgM) from the Sera of Certain Mammalian Species

1 January 1974

journal article
research article
Published by Taylor & Francis in Preparative Biochemistry

Vol. 4 (1) , 31-46
https://doi.org/10.1080/00327487408068184

Abstract

The purification of normal IgM is typically accomplished using a combination of euglobulin precipitation, gel filtration and electrophoresis or ion-exchange chromatography. The euglobulin fraction of serum contains many macroglobulins, including IgM, and is readily precipitated from serum by dialysis against distilled water¹. Mandena et al.² and Martin³ have published data that relate the solubility of IgM to ionic strength. Solubility is also affected by pH and temperature⁴. Separation of IgM from most other euglobulins may be accomplished by gel filtration⁴, chromatography on DEAE cellulose⁵, and zonal electrophoresis on starch, Pevikon⁶ or agar⁷.

Keywords

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