Antibodies against Pseudomonas aeruginosa chromosomal β-lactamase in patients with cystic fibrosis are markers of the development of resistance of P. aeruginosa to β-lactams

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Abstract
Chromosomal β-lactamase production is considered to be the most important resistance mechanism of Pseudomonas aeruginosa against β-lactams. Recently we have detected serum and sputum antibodies against P. aeruginosa chromosomal β-lactamase (aβab), using immunoblotting techniques. In this study we have developed an enzyme-linked-immunosorbent assay to measure serum aβab response in 124 cystic fibrosis patients in a cross-sectional study and in 54 cystic fibrosis patients in a longitudinal study. The aβab response occurred after a median of 3 years following onset of chronic infection and was significantly higher (PP. aeruginosa is a specific marker for resistance development of P. aeruginosa to β-lactams.