Secondary structure of the outer membrane proteins OmpA of Escherichia coli and OprF of Pseudomonas aeruginosa
- 1 October 1996
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 178 (20) , 6067-6069
- https://doi.org/10.1128/jb.178.20.6067-6069.1996
Abstract
When purified without the use of ionic detergents, both OmpA and OprF proteins contained nearly 20% alpha-helical structures, which disappeared completely upon the addition of sodium dodecyl sulfate. This result suggests that the proteins fold in a similar manner, with an N-terminal, membrane-spanning beta-barrel domain and a C-terminal, globular, periplasmic domain.Keywords
This publication has 20 references indexed in Scilit:
- An alternative topological model for Escherichia coli OmpAProtein Science, 1996
- OmpA protein of Escherichia coli outer membrane occurs in open and closed channel forms.Journal of Biological Chemistry, 1994
- Linker-insertion mutagenesis ofPseudomonas aeruginosaouter membrane protein OprFMolecular Microbiology, 1993
- Refolding and oriented insertion of a membrane protein into a lipid bilayer.Proceedings of the National Academy of Sciences, 1992
- Identification and characterization of porins in Pseudomonas aeruginosa.Journal of Biological Chemistry, 1991
- In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli.Proceedings of the National Academy of Sciences, 1990
- Models for the structure of outer-membrane proteins of Escherichia coli derived from raman spectroscopy and prediction methodsJournal of Molecular Biology, 1986
- Identification of the protein producing transmembrane diffusion pores in the outer membrane of Pseudomonas aeruginosa PA01Biochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- Circular dichroic analysis of protein conformation: Inclusion of the β-turnsAnalytical Biochemistry, 1978
- The Major Proteins of the Escherichia coli Outer Cell Envelope Membrane. Characterization of Proteins II* and III, Comparison of All ProteinsEuropean Journal of Biochemistry, 1975