Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7
- 30 November 2007
- journal article
- crystallization communications
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Vol. 63 (12) , 1073-1076
- https://doi.org/10.1107/s1744309107060289
Abstract
Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to 3.0 Å resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 Å in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.Keywords
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