Chimeric alpha-beta oligonucleotides as antisense inhibitors of reverse transcription
- 7 March 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 361 (1) , 41-45
- https://doi.org/10.1016/0014-5793(95)00138-y
Abstract
Alpha-beta chimeric 17-mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized. The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha-beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes. All chimeras inhibited in vitro cDNA synthesis in a cell-free system to various extent, via the degradation of the RNA target by RNase H.Keywords
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