A combined PAS‐myofibril stain for demonstration of early lesions of striated muscle

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Abstract
SUMMARY: To facilitate studies of early myocardial lesions a method was developed for simultaneous demonstration of saccharides and muscle proteins. Deparaffinized sections were treated consecutively with the PAS reaction, tannic acid, phosphomolybdic acid and Levanol fast cyanine 5RN. A and Z bands in normal muscle were stained blue. I bands were coloured yellow. Under various pathological conditions A and Z bands lost their affinity for Levanol fast cyanine 5RN, with and without appearance of PAS‐positive material in the Z bands.Comparison of staining patterns with chemical, electron microscopic and X‐ray diffraction data indicated that Levanol fast cyanine 5RN is bound by myosin and tropomyosin B. This affinity is lost when the protein structure is altered. The origin of the PAS‐positive material appearing in the Z bands could not yet be determined.