ACTIVATION OF N-ACETOXY-2-ACETYLAMINOFLUORENE AND N-HYDROXY-2-ACETYLAMINOFLUORENE TO MUTAGENIC AND CYTO-TOXIC METABOLITES BY V79 CHINESE-HAMSTER CELLS
- 1 January 1985
- journal article
- research article
- Vol. 149 (2) , 265-269
Abstract
N-Acetoxy-2-acetylaminofluorene (AAAF) and N-hydroxy-2-acetylaminofluorene (OH-AAF) are mutagenic to V79 cells, causing the induction of 6-thioguanine-resistant clones, and are cytotoxic. The presence of the deacetylase inhibitor, paraoxon, drastically reduces both the mutagenic and cytotoxic effects. Evidently, deacetylated metabolites are the major active species. When Salmonella typhimurium TA98 is used as target organism, addition of homogenate of V79 cells strongly potentiates the mutagenicity of OH-AAF. This is the 1st report demonstrating a significant biological effect due to the metabolism of a mutagen by V79 cells.This publication has 7 references indexed in Scilit:
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