Heterologous Adoptive Cutaneous Anaphylaxis: a Method for Detecting Reaginic Antibody Formation by Cells of the Mouse
Open Access
- 1 August 1973
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 111 (2) , 638-640
- https://doi.org/10.4049/jimmunol.111.2.638
Abstract
One approach to a rational therapy of allergic reactions is the acquisition of a greater knowledge of the factors which control reaginic antibody synthesis. We now report a method for detecting cells producing reaginic antibody in the mouse. Waltzer and Glazer first demonstrated local passive cutaneous sensitization of normal individuals by the intracutaneous injection of viable leukocytes obtained from atopic patients (1). Our procedure is based on the subsequent findings of Chase (2), Rosenberg et al. (3), and Battisto (4) that spleen, thymus, lymph node, and peritoneal exudate cells from immunized guinea pigs could be transferred to the skin of isologous or homologous guinea pigs with the subsequent detection of antibody at the site of the deposited cells. Presumably antibody from the transferred cells bound to skin mast cells and a typical passive cutaneous anaphylaxis (PCA)3 (5) reaction resulted upon injection of antigen and Evans blue dye. The technique was termed adoptive cutaneous anaphylaxis (ACA) by Battisto (4).Keywords
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