Haplotyping by double PCR amplification of specific alleles.

  • 1 April 1991
    • journal article
    • Vol. 10  (4)
Abstract
Haplotypes are useful in population genetics and medicine. Determining haplotypes in the absence of DNA samples from appropriate family members can be difficult and laborious. We have developed a rapid and reproducible method for haplotyping an individual in the absence of relatives. The method utilizes pairs of allele-specific PCR primers to differentially amplify each haplotype. Four amplifications can distinguish the haplotypes produced by two biallelic polymorphisms.

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