Gas-Liquid Chromatographic Isolation, Identification, and Quantitation of Some Barbiturates, Glutethimide, and Diphenylhydantoin in Whole Blood
- 1 November 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 18 (11) , 1343-1349
- https://doi.org/10.1093/clinchem/18.11.1343
Abstract
An accurate method is described for the qualitative and quantitative determination of 11 barbiturates, glutethimide, and diphenylhydantoin in a single extract obtained from 1 ml of whole blood, which is extracted with ether at pH 2 and separately but simultaneously gas-liquid chromatographed on a selective (3% OV-17) and a nonselective (5% OV-1) column. Retention data are given. Results for barbiturate obtained by the Broughton [Biochem. J.63, 207 (1956)] spectrophotometric method and this gas-liquid chromatographic method are compared. Blood from more than 30 patients has been analyzed, and data are given to illustrate some of the findings.Keywords
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