The Effect of Complement in Rabbit Serum on the Quantitative Precipitin Reaction. I.

Open Access
Publisher Website
Google Scholar
Abstract
Summary and Conclusions: Normal rabbit serum and low titered antisera contain appreciable quantities of complement nitrogen even after 3 to 5 months of storage at 0 C. Significant differences in both the total nitrogen precipitated from antisera with low concentrations of antibody and the antibody N/antigen N ratios before and after decomplementation of the antisera have been demonstrated. The differences in immunochemical behavior of antisera diluted in normal rabbit serum and in 0.85% saline are markedly reduced after decomplementation of the diluting serum.