HPLC-MSn Analysis of Phenolic Compounds and Purine Alkaloids in Green and Black Tea
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- 27 April 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 52 (10) , 2807-2815
- https://doi.org/10.1021/jf0354848
Abstract
Tea is a complex mixture containing a range of compounds from simple phenolics to complex thearubigins, many of which have well-recognized antioxidant properties. This paper describes the application of high-performance liquid chromatography−mass spectrometry (HPLC-MSn) methods for the rapid and routine analysis of more than 30 phenolics in tea. Green and black tea infusions were injected directly onto a reversed phase HPLC column, and the phenolics eluted using two different mobile phase gradients, one optimized to resolve catechin derivatives and the other, flavonols and theaflavins. Compounds, identified on the basis of their retention time, absorbance spectrum, and MS fragmentation pattern, included (+)-catechin, (−)-epicatechin, theaflavin and their various gallate derivatives, quercetin and kaempferol mono-, di-, and triglycosides, quinic acid esters of gallic acid and hydroxycinnamates, and the purine alkaloids, caffeine and theobromine. Keywords: Camellia sinensis; green tea; black tea; HPLC-MSn analysis; catechins; theaflavins; flavonols; gallates; quinic acid derivatives of hydroxycinnamates; purine alkaloidsKeywords
This publication has 14 references indexed in Scilit:
- Determination of Tea Components with Antioxidant ActivityJournal of Agricultural and Food Chemistry, 2003
- Identification of Two Novel Proanthocyanidins in Green TeaJournal of Agricultural and Food Chemistry, 1999
- [15] Size separation of condensed tannins by normal-phase high-performance liquid chromatographyPublished by Elsevier ,1999
- Simultaneous analysis of individual catechins and caffeine in green teaPublished by Elsevier ,1998
- Thermospray-LC-MS analysis of various groups of polyphenols in teaZeitschrift für Lebensmittel-Untersuchung und Forschung, 1996
- Chromatography of tea constituentsJournal of Chromatography A, 1992
- Determination of flavanols, theogallin, gallic acid and caffeine in tea using HPLCZeitschrift für Lebensmittel-Untersuchung und Forschung, 1991
- Flavonol glycosides in tea—kaempferol and quercetin rhamnodiglucosidesJournal of the Science of Food and Agriculture, 1991
- Flavonol triglycosides containing galactose in teaPhytochemistry, 1991
- The identification of theogallin as 3-galloylquinic acidPhytochemistry, 1971