Digital Readout of Target Binding with Attomole Detection Limits via Enzyme Amplification in Femtoliter Arrays
- 20 April 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (19) , 6286-6287
- https://doi.org/10.1021/ja058425e
Abstract
In this communication, single molecules of β-galactosidase were captured on a 1 mm femtoliter array using biotin−streptavidin binding. The femtoliter arrays, containing 24 000 individual reaction chambers, permit digital concentration readout as the percentage of reaction vessels that successfully capture a target molecule is correlated to the bulk target concentration. This capture and readout approach should prove useful for DNA and antibody assays that utilize an enzyme label to catalyze the generation of a fluorescent signal.Keywords
This publication has 12 references indexed in Scilit:
- Digital Concentration Readout of Single Enzyme Molecules Using Femtoliter Arrays and Poisson StatisticsNano Letters, 2006
- Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplificationTrends in Biotechnology, 2005
- Microfabricated arrays of femtoliter chambers allow single molecule enzymologyNature Biotechnology, 2005
- The 'right' size in nanobiotechnologyNature Biotechnology, 2003
- Particle Formation by Ion Nucleation in the Upper Troposphere and Lower StratosphereScience, 2003
- Single-Molecule Measurement of Protein Folding KineticsScience, 2003
- Single-molecule PCR using water-in-oil emulsionJournal of Biotechnology, 2003
- High-throughput PCR in silicon based microchamber arrayBiosensors and Bioelectronics, 2001
- Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCRNucleic Acids Research, 1999
- Nonequilibrium Self-Assembly of Long Chains of Polar Molecules in Superfluid HeliumScience, 1999