Thermal Tolerance ofMycobacterium paratuberculosis
- 1 March 1998
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 64 (3) , 999-1005
- https://doi.org/10.1128/aem.64.3.999-1005.1998
Abstract
Dvalues (decimal reduction time; the time required to kill 1 log concentration of bacteria) were determined for both human and bovine strains (Dominic, Ben, BO45, and ATCC 19698) ofMycobacterium paratuberculosisin 50 mM lactate solution (pH 6.8) and in milk at four temperatures (62, 65, 68, and 71°C). ViableM. paratuberculosisorganisms were quantified by a radiometric culture method (BACTEC). Thermal death curves for theM. paratuberculosisstrains tested were generally linear, withR2of ≥0.90, but a few curves (R2, 0.80 to 0.90) were better described by a quadratic equation. The human strains (Dominic and Ben) had similarDvalues in milk and in lactate solution. However,Dvalues for the bovine strains (BO45 and ATCC 19698) were significantly different depending on the menstruum.Dvalues for low-passage clinical strains (Dominic, Ben, and BO45) were lower than those of the high-passage laboratory strain (ATCC 19698). TheDvalue based on pooled data for clinical strains ofM. paratuberculosisin milk at 71°C (D71°C) was 11.67 s. PooledD62°C,D65°C, andD68°Cof clinicalM. paratuberculosisstrains in milk were 228.8, 47.8, and 21.8 s, respectively. TheZvalue (the temperature required for the decimal reduction time to traverse 1 log cycle) of clinical strains in milk was 7.11°C. TheDvalues of clumped and singleM. paratuberculosiscells were not significantly different. TheDvalues of allM. paratuberculosisstrains tested were considerably higher than those published forListeria,Salmonella, andCoxiellaspp. and estimated forMycobacterium bovis, indicating thatM. paratuberculosisis more thermally tolerant. This study supports the premise thatM. paratuberculosismay survive high-temperature, short-time pasteurization when the initial organism concentration is greater than 101cells/ml.Keywords
This publication has 39 references indexed in Scilit:
- The role of mycobacteria in Crohn's diseaseJournal of Medical Microbiology, 1994
- Detection of Mycobacterium paratuberculosis by Polymerase Chain Reaction in Children with Crohn's DiseaseThe Journal of Infectious Diseases, 1994
- Mycobacterium paratuberculosis in Intestinal Tissue from Patients with Crohn's Disease Demonstrated by a Nested Primer Polymerase Chain ReactionScandinavian Journal of Gastroenterology, 1994
- The Thermal Resistance of Mycobacterium Paratuberculosis in Raw Milk under Conditions Simulating PasteurizationJournal of Veterinary Diagnostic Investigation, 1993
- Mycobacterium paratuberculosis DNA in Crohn's disease tissue.Gut, 1992
- Specific detection of Mycobacterium paratuberculosis by DNA hybridisation with a fragment of the insertion element IS900.Gut, 1991
- Thermoresistance of MycobacteriaActa Veterinaria Brno, 1990
- Preliminary report on isolation of mycobacteria from patients with Crohn's diseaseDigestive Diseases and Sciences, 1989
- Possible role of mycobacteria in inflammatory bowel diseaseDigestive Diseases and Sciences, 1984
- Pasteurization of Milk Containing the Organism of Q FeverAmerican Journal of Public Health and the Nations Health, 1957