Effect of hydrogen peroxide on the metabolism of articular chondrocytes
- 1 July 1999
- journal article
- research article
- Published by Springer Nature in Inflammation Research
- Vol. 48 (7) , 399-403
- https://doi.org/10.1007/s000110050478
Abstract
Objective: To examine the effect of hydrogen peroxide on chondrocyte metabolism.¶Materials and Methods: Bovine articular chondrocytes were used. Proteoglycan (PG) synthesis was measured with [35S] sulfate incorporation. For detection of apoptosis, the TdT-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V assay were used. Extracellular-regulated protein kinase (ERK) activity was measured using a mitogen-activated protein kinase assay system.¶Results: Addition of hydrogen peroxide resulted in the inhibition of PG synthesis, apoptosis, and enhanced ERK activity.¶Conclusion: Hydrogen peroxide plays an important role in regulating the metabolism of chondrocytes.Keywords
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