Zebrafish pax[b] is involved in the formation of the midbrain–hindbrain boundary
- 1 November 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 360 (6399) , 87-89
- https://doi.org/10.1038/360087a0
Abstract
Among the genes thought to be involved in patterning the nervous system are a family of developmentally regulated paired box-containing (Pax) genes. Mutations in some of these Pax genes lead to severe developmental abnormalities. Zebrafish pax[b](pax[zf-b]) is a member of the Pax gene family that is expressed in the presumptive posterior midbrain from the end of gastrulation and, at later stages, in other localized regions of the developing embryo. Here we show that injection of antibodies raised against the pax[b] protein causes a localized malformation at the midbrain-hindbrain boundary. In situ hybridizations demonstrate that antibody injection causes downregulation of pax[b] transcripts in the posterior midbrain and alteration of wnt-1 and eng-2 expression in this area. The data demonstrate an involvement of pax[b] in the formation of the midbrain-hindbrain junction.Keywords
This publication has 35 references indexed in Scilit:
- The paired domain‐containing nuclear factor Pax[b] is expressed in specific commissural interneurons in zebrafish embryosJournal of Neurobiology, 1992
- An exonic mutation in the HuP2 paired domain gene causes Waardenburg's syndromeNature, 1992
- Waardenburg's syndrome patients have mutations in the human homologue of the Pax-3 paired box geneNature, 1992
- Mouse Small eye results from mutations in a paired-like homeobox-containing geneNature, 1991
- Positional cloning and characterization of a paired box- and homeobox-containing gene from the aniridia regionCell, 1991
- Pax: A murine multigene family of paired box-containing genesGenomics, 1991
- The molecular basis of the undulated/Pax-1 mutationCell, 1991
- The paired box encodes a second DNA-binding domain in the paired homeo domain protein.Genes & Development, 1991
- The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular systemMechanisms of Development, 1990
- Pax 1, a member of a paired box homologous murine gene family, is expressed in segmented structures during developmentCell, 1988