Distribution and Fate of 4-C14-Cholesterol in the Adult Male American Cockroach
- 1 January 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of the Entomological Society of America
- Vol. 56 (1) , 115-119
- https://doi.org/10.1093/aesa/56.1.115
Abstract
The distribution and fate of 4-C14-cholesterol was studied in adult male American cockroaches (Peripluneta americana (L.)) at 1, 10, and 20 days following injection. More than 80% of the administered radioactivity was still present in the cockroaches at the end of 20 days, indicating a strict sterol economy. Exchange and transport of 4-C14-cholesterol between organs and tissues were evidenced by the continuing presence of radioactive compounds in the hemolymph and changes in the concentration of C14 compounds in several tissues. Paper chromatographic analyses of extracts indicated the presence of free sterols, sterol esters, and more polar steroids in all the tissues examined. Free sterols were predominant, accounting for 55% to 98% of the radioactive compounds present. The highest percentage of radioactive sterol esters (44%) was found in the fat body. Polar steroids were found in low concentrations except in the midgut and hindgut, where they accounted for more than one-tenth of the total radioactivity. When the C14 sterols isolated from whole insects were analyzed by gas-liquid chromatography and reverse isotope dilution, greater than 97% of radioactivity was found to behave like unchanged cholesterol.Keywords
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