SIMULTANEOUS DETERMINATION OF TRYPTOPHAN AND ITS 5-HYDROXY METABOLITES IN HUMAN CEREBROSPINAL-FLUID BY REVERSED PHASE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION
- 1 January 1983
- journal article
- research article
- Vol. 43 (6) , 463-472
Abstract
A sensitive ion-air reversed phase liquid chromatographic method for determining tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine and 5-hydroxyindole acetic acid was developed. To separate these indoles in CSF both octadecyl- and phenyl-bonded columns were tested. The effect of an ion-pair reagent (n-heptyl sulphonate) and pH of the mobile phase on the separation was examined. With the method described the concentrations of the 4 indoles can be determined within 45 min without sample pretreatment. The sensitivity obtained with electrochemical detection is 0.45 .mu.mol/l for tryptophan and 2-4 nmol/l for 5-hydroxyindoles in human CSF samples.This publication has 2 references indexed in Scilit:
- Optimized isocratic conditions for the simultaneous determination of serotonin precursors and metabolites by reversed-phase high-pressure liquid chromatography with electrochemical detectionAnalytical Biochemistry, 1981
- Reversed-phase high performance liquid chromatography of catecholamines and their congeners with simple acids as ion-paring reagentsJournal of Chromatography A, 1979