In vivo and In vitro Excystation of Zygocotyle lunata (Trematoda) Metacercariae and Histochemical Observations on the Cyst

Publisher Website
Google Scholar
Abstract
Encysted metacercariae of Z. lunata (Trematoda) excyst within 2 h postexposure in the lower ileum of the domestic chick. Optimal in vitro excystation of this species occurs following pretreatment of the cyst for 15 min in 1% acidified pepsin, treatment in 0.02 M sodium dithionite (a reductant) for 1-2 min and then 2 h treatment in an excystation medium containing 1% sodium glycocholate plus 1% trypsin in Earle''s BSS [balanced salt solution] adjusted to pH 8.8 with Tris and maintained at 41.degree. C. The cyst of this species is a dome-shaped hemisphere containing an inner and outer wall. The outer wall contains mainly acid mucopolysaccharides, whereas the inner wall is mainly proteinaceous. The cyst contains a ventral lid which only was seen during excystation.

This publication has 0 references indexed in Scilit: