Accurate measurement of molar absorptivities
- 1 January 1972
- journal article
- Published by National Institute of Standards and Technology (NIST) in Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry
- Vol. 76A (5) , 483-489
- https://doi.org/10.6028/jres.076a.042
Abstract
The key to accurate measurement of molar absorptivities is a thorough understanding of the sources of error which appear throughout the measurement procedure. Sources of determinant error will be listed with comments on estimating their magnitude and eliminating them where possible. Sources of random error will be discussed as well as the propagation of both random and determinant errors. There is discussion of the need for accurate values of molar absorptivities using examples from clinical chemistry. Finally, the proper use of accurate absorptivity values in the clinical chemistry laboratory will be considered. Here, emphasis is on the need for a quality assurance system which includes routine checks on such things as wavelength calibration and photometric accuracy of spectrophotometers, calibration of analytical balances, and quality of incoming reagents.Keywords
This publication has 3 references indexed in Scilit:
- Factors Affecting the Development of the Clinical Laboratory SciencesClinical Chemistry, 1971
- Practical Spectrophotometric StandardsClinical Chemistry, 1969
- THE EXTINCTION COEFFICIENTS OF THE REDUCED BAND OF PYRIDINE NUCLEOTIDESJournal of Biological Chemistry, 1948