Decalcification and Staining of Archaeological Bones, With Histochemical Interpretation of Metachromasia
- 1 January 1960
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 35 (2) , 91-96
- https://doi.org/10.3109/10520296009114722
Abstract
Metachromatic staining with azure A and toluidine blue was achieved on decalcified archaeological bones. The material was fixed in 10% formalin and in absolute alcohol and paraffin sections were stained in 0.1% azure A in 30% ethanol as well as in 0.5% aqueous toluidine blue. Various methods of decalcification are compared, and the authors recommend decalcification in equal parts of 2% citric acid and 20% sodium citrate, pH about 6.Keywords
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