Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin

Open Access

12 November 1984

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 12 (21) , 7987-8000
https://doi.org/10.1093/nar/12.21.7987

Abstract

A cDNA library from membrane-bound poly(A)⁺ mRNA of developing potato tubers was constructed and two classes of essentially full-length cDNA clones for a precursor to the major tuber glycoprotein, patatin were isolated. Sequence analysis shows that the two classes are approximately 98% homologous and correspond to the two major species of patatin identified previously by NH₂-terminal amino acid sequence analysis. Sequence analysis also predicts that patatin 1s synthesized with a 23 amino acid signal sequence. Northern blot analysis shows that patatin mRNAs are 155O±5O nucleotides 1n length and are normally not present in polyribosomal or total RNA from stems or leaves.

Keywords

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