Caenorhabditis elegans PMR1, a P‐type calcium ATPase, is important for calcium/manganese homeostasis and oxidative stress response

Abstract
The Caenorhabditis elegans PMR1, a P‐type Ca2+/Mn2+ ATPase, is expressed in hypodermal seam cells, intestinal cells and spermatheca; localized in Golgi complex. Knock down of pmr‐1 as well as overexpression of truncated Caenorhabditis elegans PMR1, which mimics dominant mutations observed in human Hailey–Hailey disease, renders the worm highly sensitive to EGTA and Mn2+. Interestingly, pmr‐1 knock down not only causes animals to become resistant to oxidative stress but also suppresses high reactive oxygen species sensitivity of smf‐3 RNA‐mediated interference and daf‐16 worms. These findings suggest that C. elegans PMR1 has important roles in Ca2+ and Mn2+ homeostasis and oxidative stress response.