Histo-Immunological Detection of Collagen Fractions

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Abstract
Summary The soluble and insoluble fractions of collagen from chicken leg tendon were used as antigens to induce corresponding antibodies in adult rabbits and guinea pigs. Antibodies were checked immuno-sero-logically and the globulins or its gamma fraction labeled with fluorescein isothiocyanate and purified with Sephadex. Adequately controlled Coons' technique was used in order to detect the presence of the antigen in different types of connective tissue of adult chickens. It was observed: 1) as previously demonstrated, there was cross reaction between the neutral-soluble and the citrate-soluble fractions, whereas a direct reaction occurred with the insoluble collagen fraction, 2) the fluorescent antibodies against these different fractions reacted similarly with the collagen fibers and bundles but not with reticulin and elastic fibers, mucopolysaccharides, basement membranes, nor with matrix of cartilage and bone, 3) intensity of fluorescence was higher with the fibers of dense connective tissue as compared with loose or mucoid type of connective tissue.

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