Liquid Chromatographic-Fluorometric Analysis of Paralytic Shellfish Toxins

Abstract
Alkaline oxidation of paralytic shellfish toxins with ten-butyl hydroperoxide yielded highly fluorescent derivatives. Even gonyautoxins I and IV and neosaxitoxin, which were nonfluorescing by previously proposed hydrogen peroxide oxidations, were successfully converted to fluorescent compounds. A continuous shellfish toxin analyzer was constructed by incorporating the reaction into a high pressure liquid chromatographic system. The limit of detection was within the range of 0.04 ~ 2.2 nmol for gonyautoxins I ~ V, neosaxitoxin and saxitoxin.

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