The synthesis of oligoribonucleotides V. The stepwise synthesis of the 3′-terminal heptanucleotide sequence of tRNAfMet from E. coli
- 1 December 1979
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Chemistry
- Vol. 57 (23) , 3140-3144
- https://doi.org/10.1139/v79-512
Abstract
The stepwise synthesis of a heptaribonucleotide GpCpApApCpCpA corresponding to the 3′-terminus of tRNAfMet from E. coli is described. The procedure takes advantage of the ease of obtaining ribonucleoside units bearing a tert-butyldimethylsilyl group on the 2′-position. These units, bearing a 5′-methoxytrityl group are phosphorylated at the 3′-position and coupled to the free 5′-position of the growing nucleotide chain using the chlorophosphite coupling procedure. Yields varied from 87 to 50% over the entire sequence.This publication has 8 references indexed in Scilit:
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