Subcellular Distribution of the Isoenzymes of N ADP Isocitrate Dehydrogenase in Rat Liver and Heart
- 1 July 1968
- journal article
- Published by S. Karger AG in Enzymologia Biologica Et Clinica
- Vol. 9 (5) , 393-399
- https://doi.org/10.1159/000458268
Abstract
Summary. The sub-cellular distribution of the isoenzymes of NADP isocitrate dehydrogenase (NADP-L(S) isocitrate oxidoreductase, 1.1.1.42) in rat heart and liver has been investigated. In heart the soluble fraction contained both fast and slow NADP isocitrate dehydrogenase isoenzymes and the microsomal, mitochondrial and nuclear fractions contained only the slow iso-enzyme. In liver the soluble fraction contained the fast NADP isocitrate dehydrogenase isoenzyme only, the microsomal and mitochondrial fractions contained both fast and slow isoenzymes while the nuclear fraction contained only the slow form. Experiments on the distribution and recovery of total NADP isocitrate dehydrogenase activity in unpurified sub-cellular fractions showed that heart tissue contained a higher activity in the nuclear and mitochondrial fractions than did the liver, and the liver had a higher activity in the soluble fraction than did the heart.Keywords
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