Dimethyl sulfoxide as an inducer of differentiation in preosteoblast MC3T3‐E1 cells
- 6 December 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (1) , 121-126
- https://doi.org/10.1016/j.febslet.2005.11.062
Abstract
Osteoblastic differentiation is an essential part of bone formation. Dimethyl sulfoxide (DMSO) is a water miscible solvent that is used extensively for receptor ligands in osteoblast studies. However, little is known about its effects on osteoblastogenic precursor cells. In this study, we have used a murine preosteoblast cell line MC3T3-E1 cells to demonstrate that DMSO effectively induces osteoblastic differentiation of MC3T3-E1 cells via the activation of Runx2 and osterix and is dependent upon the protein kinase C (PKC) pathways. We further demonstrated that prolonged activation of PKC pathways is sufficient to induce osteoblastic differentiation, possibly via the activation of PKD/PKCmu.Keywords
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