Conjugation between bacterial and mammalian cells
- 19 November 2001
- journal article
- research article
- Published by Springer Nature in Nature Genetics
- Vol. 29 (4) , 375-376
- https://doi.org/10.1038/ng779
Abstract
Bacterial conjugation, in which DNA is transferred from one bacterium to another, was first reported in 1946 and found to be mediated by the F factor1. Although the F and RK2/RP4 prototypic plasmids can mediate the transfer of DNA from bacteria to yeast2, there has been no evidence of classical bacterial conjugation to higher eukaryotes. Here, I present evidence of such transfer, using Escherichia coli, the RK2 plasmid system and Chinese hamster ovary CHO K1 cells.Keywords
This publication has 15 references indexed in Scilit:
- Genetic transformation of HeLa cells by AgrobacteriumProceedings of the National Academy of Sciences, 2001
- Agrobacterium tumefaciens-mediated transformation of yeast.Proceedings of the National Academy of Sciences, 1996
- Different relative importances of the par operons and the effect of conjugal transfer on the maintenance of intact promiscuous plasmid RK2Journal of Bacteriology, 1995
- A Region of Human CD14 Required for Lipopolysaccharide BindingJournal of Biological Chemistry, 1995
- The yeast Saccharomyces kluyveri as a recipient eukaryote in transkingdom conjugation: behavior of transmitted plasmids in transconjugantsJournal of Bacteriology, 1994
- Retrotransfer of IncP piasmid R751 from Escherichia coli maxicells: evidence for the genetic sufficiency of self‐transferable plasmids for bacterial conjugationMolecular Microbiology, 1993
- Mutational analysis of essential IncP alpha plasmid transfer genes traF and traG and involvement of traF in phage sensitivityJournal of Bacteriology, 1992
- Sequence identity in the nick regions of IncP plasmid transfer origins and T-DNA borders of Agrobacterium Ti plasmids.Proceedings of the National Academy of Sciences, 1991
- Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeastNature, 1989
- Gene Recombination in Escherichia ColiNature, 1946