On Preparative Separation of Brain Mitochondrial Monoamine Oxidases

Abstract
A method was developed for solubilization from bovine brain stem mitochondrial fraction of monoamine oxidases deaminating biogenic amines. Preparative separation of the monoamine oxidases, possessing different substrate specificities, was achieved by column chromatography on a biospecific adsorbent AH-Sepharose 4 B. The enzyme parparations did not contain any detectable by disc-electrophoresis or isoelectrofocusing in polyacrylamide gels proteins which were devoid of the monoamine oxidase activity.