The Percentages of HB F and of Gγ and Aγ Chains in the Hb F Synthesized by Reticulocytes and BFUe-Derived Colonies of Patients with Sickle Cell Anemia
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 6 (4) , 369-378
- https://doi.org/10.3109/03630268208996942
Abstract
The synthesis of Hb F was studied in the BFUe-derived colonies of 20 SS patients after incubation with 35s methionine for 24 hours, 13 days after the start of the experiments. Similar analyses were made for isolated reticulocytes from 28 SS patients; these cells were incubated for 2 hours and occasionally for 24 hours. The Gγ and Aγ percentages were determined in these two Hb F preparations and in the Hb F of circulating red cells using two different HPL chromatographic procedures. The data show an increased production of Hb F in the colonies mainly of patients with low blood Hb F values. A close correlation was present between the % Gγ chain in peripheral red cells and the Gγ percentages observed for the Hb F synthesized in the BFUe-derived colonies and that in reticulocytes. However, the values for the reticulocytes were significantly higher than those for the colonies. These data indicate a considerable variability among SS patients in their ability to produce Hb F in in vitro colonies which may in part be due to differences in the numbers of BFUe types in circulation. The variation in Gγ and Aγ levels in Hb F synthesized in reticulocytes and BFUe-derived colonies could be the result of slight differences in the stability of the corresponding mRNA's.This publication has 21 references indexed in Scilit:
- Stochastic expression of fetal hemoglobin in adult erythroid cells.Proceedings of the National Academy of Sciences, 1981
- In vitro synthesis of hemoglobin and hemoglobin chains in the BFUe-derived colonies from persons with α- or β-thalassemiaAmerican Journal of Hematology, 1981
- Asynchronous synthesis of HbF and HbA during erythroblast maturation. II. Studies of G gamma, A gamma, and beta chain synthesis in individual erythroid clones from neonatal and adult BFU-E culturesBlood, 1981
- The synthesis of theGγ andAγ chains of human fetal hemoglobin in erythroid colonies cultured from peripheral blood BFUe's of normal adults and newborn and of subjects with anAγ or aGγ chain abnormal fetal hemoglobinAmerican Journal of Hematology, 1980
- Hemoglobin synthesis in individual bursts from normal adult blood: all bursts and subcolonies synthesize G gamma-and A gamma-globin chainsBlood, 1980
- Adult and Fetal Hemoglobin Production in Erythroid Colonies from Subjects with β-Thalassemia or with Hereditary Persistence of Fetal Hemoglobin (HPFH)Hemoglobin, 1980
- Cellular regulation of hemoglobin switching: evidence for inverse relationship between fetal hemoglobin synthesis and degree of maturity of human erythroid cells.Proceedings of the National Academy of Sciences, 1979
- The Synthesis of Fetal Hemoglobin Types in red Blood Cells and in BFU-E Derived Colonies from Peripheral Blood of Patients with Sickle Cell Anemia, β+- and δβ-Thalassemia, Various forms of Hereditary Persistence of Fetal Hemoglobin, Normal Adults and NewbornHemoglobin, 1979
- Augmentation of fetal hemoglobin (HbF) synthesis in culture by human erythropoietic precursors in the marrow and peripheral blood: studies in sickle cell anemia and nonhemoglobinopathic adultsBlood, 1978
- Erythroid Progenitors Circulating in the Blood of Adult Individuals Produce Fetal Hemoglobin in CultureScience, 1978