Improved Techniques for Assessment of Serum Lipoprotein Patterns. II. Rapid Method for Diagnosis of Type III Hyperlipoproteinemia without Ultracentrifugation
Open Access
- 1 October 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 19 (10) , 1139-1141
- https://doi.org/10.1093/clinchem/19.10.1139
Abstract
Based on the previously described technique [Clin. Chem.19, 737 (1973)] of precipitating plasma lipoproteins with polyanions after their electrophoretic separation in gels, a new method is presented for diagnosing type III hyperlipoproteinemia without need for ultracentrifugation or immunologic techniques. Used in the procedure are 0.1 mol/liter MgCl2, 1.5 g/liter heparin, and 10 g/liter NaCl to visualize selectively the very-low-density lipoproteins in agarose gel after electrophoresis. The technique is simple, inexpensive, accessible to every laboratory, and provides the answer in less than 2 h after electrophoresis of the patient’s whole serum. The results obtained are the same as those obtained by ultracentrifugation followed by lipoprotein electrophoresis of the isolated fractions.Keywords
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