INDUCTION OF SQUAMOUS-CELL CARCINOMA IN LUNG OF C57BL-6 MICE BY INTRATRACHEAL INSTILLATION OF BENZO[A]PYRENE WITH CHARCOAL POWDER

  • 1 January 1977
    • journal article
    • research article
    • Vol. 68  (3) , 343-352
Abstract
Squamous cell carcinomas were readily induced in the lung of C57BL/6 mice by repeated intratracheal instillations of benzo[a]pyrene with charcoal powder suspended in saline solution. Intratracheal instillations were performed once a week for 8 wk, and the observation period of the experiment was 40 wk. Some of the tumors were highly keratinized and others were moderately keratinized squamous cell carcinomas. At a later period of observation, poorly differentiated squamous cell carcinomas were found. Invasion into blood vessels was occasionally seen but metastases to regional lymph nodes or distant metastases was not found. One tumor from a mouse killed on the 280th day was successfully transplanted s.c. into C57BL/6 mice. This mouse, killed on the 84th day after the 1st transplantation, had a pulmonary metastases.