Evidence of extrachromosomal forms of hepatitis B viral DNA in a bone marrow culture obtained from a patient recently infected with hepatitis B virus.

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Abstract
A cell culture that produces Dane-like particles was initiated from a bone marrow aspirate of an acute hepatitis B patient. By using Southern blot analysis and a recombinant hepatitis B virus (HBV) DNA plasmid probe, extrachromosomal forms of HBV DNA were detected. The 2 forms of HBV DNA migrate as a closed circular 2.2-kb kilobase form and an open circular 3.9-kb form. There was no evidence of HBV DNA integration into the host genome.
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