A Simplified Method for the In vitro Cultivation of the Rat Tapeworm, Hymenolepis diminuta
- 1 August 1965
- journal article
- research article
- Published by JSTOR in Journal of Parasitology
- Vol. 51 (4) , 516-+
- https://doi.org/10.2307/3276218
Abstract
Hymenolepis diminuta has been grown in vitro from the cysticercoid to the patent adult stage using a diffused diphasic medium consisting of a blood-agar base (NNN) overlaid with Hanks'' balanced salt solution. The culture flasks were incubated at 37 C in a Dubnoff metabolic shaking incubator under a gas mixture of 97% N2-3% CO2. Details of methodology and data on the growth and development of worms cultivated in this simplified system are reported.Keywords
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