Stability of continuous flow electrophoresis

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Abstract
The stability of continuous flow electrophoresis (CFE) was tested by performing uninterrupted continuous flow cationic isotachophoresis for 15 h, using a mixture of two synthetic isoelectric point (pI) markers as the model sample. This sample as well as the other operational electrolytes were continuously pumped into the CFE separation chamber via 10 individual inlets. The operational electrolyte system and the arrangement of the electrolyte streams were selected in accord with previously published results, providing a stable electrophoretic regime. The chamber had 96 individual outlets and here 96 individual fractions were collected in microwell plates in regular time intervals and scanned photometrically. The scanned photometric patterns proved that CFE is sufficiently stable for an overnight operation.