Thrombase—its preparation and properties

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Abstract
Thrombase may be prepared from oxalated ox blood by (a) the spontaneous activation of prothrom-base prepared in the way previously described, (b) the solution of this thrombase in 0.5% NaCl, and (c) precipitation of the thrombase by 4 volumes of acetone. 1 mgm. of thrombase coagulates 100 cc. of oxalate plasma in 30 sec. The intraven. injection of 2 mgm. into a rabbit causes intravascular coagulation and death. Thrombase is a protein, soluble in water. The enzyme, dissolved in water, slowly dialyzes through a collodion membrane. It is destroyed by 0.01 N HCl and 0.01 N NaOH. The action of thrombase on plasma shows the following relations: (a) the time of coagulation is inversely proportional to the quantity of enzyme, and (b) the dilution of a plasma thrombase soln. prolongs the time of coagulation in proportion to the dilution. When fibrinogen is converted into fibrin by thrombase a quantity of thrombase is removed from solution in proportion to its original concn. Gamgee''s method for the preparation of thrombase from fibrin (Jour. Phys. 2, 1897) depends partly upon the resolution of this adsorbed thrombase by 8% NaCl. The hypothesis is put forward that thrombase is a proteoclastic enzyme which splits fibrinogen into fibrin and serum globulin.

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