Stable Expression of a New Chimeric Fluorescent Reporter in the Human Malaria Parasite Plasmodium falciparum
- 1 April 2000
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (4) , 2328-32
- https://doi.org/10.1128/iai.68.4.2328-2332.2000
Abstract
Stable transfection of a new, chimeric reporter in the human malaria parasite Plasmodium falciparum confers green fluorescence and methotrexate resistance that can be quantitated by Western blotting and flow cytometry. This provides a sensitive, live reporter for exploitation of genomic and high-throughput assays for the identification of new pathogenic determinants.Keywords
This publication has 20 references indexed in Scilit:
- A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparumProceedings of the National Academy of Sciences, 1999
- Stable expression of green fluorescent protein in blood and mosquito stages of Plasmodium bergheiMolecular and Biochemical Parasitology, 1998
- Circumsporozoite protein is required for development of malaria sporozoites in mosquitoesNature, 1997
- Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid‐inducible promoters by differential fluorescence inductionMolecular Microbiology, 1996
- Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum.Proceedings of the National Academy of Sciences, 1996
- Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine.Proceedings of the National Academy of Sciences, 1996
- FACS-optimized mutants of the green fluorescent protein (GFP)Gene, 1996
- Stable Transfection of Malaria Parasite Blood StagesScience, 1995
- Transfection of Plasmodium falciparum within human red blood cells.Proceedings of the National Academy of Sciences, 1995
- Transfection of the malaria parasite and expression of firefly luciferase.Proceedings of the National Academy of Sciences, 1993