The application of oligonucleotide probes to HLA Class II typing of the DRB sub‐region
- 1 October 1990
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 36 (4) , 149-155
- https://doi.org/10.1111/j.1399-0039.1990.tb01821.x
Abstract
A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell‐lines selected to cover a wide range of specificities, and 40 RFLP‐typed controls. The results are presented and discussed. The simplicity and accuracy of this scheme are emphasized.Keywords
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