STUDIES ON THE GROWTH OF SPECIES OF ACTINOMYCES I

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Abstract
Thirteen strains of Actinomyces, including strains that have been described as A. israeli and A. naeslundi, were successfully grown and maintained on a liquid synthetic medium, containing purified starch, adjusted to pH 6.5. Using optical density determinations as an index of growth it was demonstrated that in most instances, after 7 days incubation, growth in the synthetic medium was as good as or better than that obtained after 8 days in a casitone yeast extract medium (modified fluid thioglycollate); the rates or amounts of growth or both of certain strains were significantly decreased by raising the pH of the medium from 6.5 to 7.0 or 7.5; and lactic acid and traces of ethanol, acetic and formic acids were formed from glucose by these organisms on this medium.