Surface Sulphydryl Groups and Hexose Monophosphate Pathway Activity in Resting Human Polymorphonuclear Leucocytes

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Abstract
Summary. The role of surface ‐SH groups on the hexose monophosphate pathway activity in resting human polymorphonuclear leucocytes was studied. Endotoxin, taurocholic acid and concanavalin A stimulated [1‐14C]glucose oxidation to a greater degree than [6‐14C]glucose oxidation. The stimulation of glucose‐1‐C oxidation by endotoxin, taurocholic acid and concanavalin A could be prevented by p‐chloromercurybenzene sulphonic acid, an inhibitor of surface ‐SH groups. In contrast, polymorphonuclear leucocytes from four patients with chronic granulomatous disease failed to show stimulation of glucose‐1‐C oxidation by endotoxin, taurocholic acid or concanavalin A.